Filtration unit with pleated 0.2 µm PES membrane for vacuum filtration / sterile filtration of up to several litres. Opening the outlet valve, which is protected from incoming contaminants by a 0.2 µm PTFE membrane, breaks the vacuum so that the full receiving container can be replaced with a new one. Filtration begins again when the outlet valve is closed. This process of 'continuous' filtration can be repeated several times. The 0.2 µm pleated PES membrane with a filtration area of 150 cm2 guarantees safe sterile filtration of media, buffers and many other solutions.