Lightning Red for 1D SDS PAGE

SERVA Lightning Red is a fluorescent dye for rapid labelling of proteins prior to 1D SDS PAGE, making any staining and washing steps after electrophoresis unnecessary. In addition the dye is fully compatible with mass spectrometry and other downstream methods like Western Blotting. The labelling procedure is simple and quick: Just mix your sample with the provided SERVA SDS sample buffer pH 8.3, after heating add the sample to 10 μl dye solution and incubate for 30 min at room temperature. After the run detection of labelled proteins is performed by fluorescent imager (camera or scanner) at an excitation wavelength of about 530 nm and emission filter of 610 nm with a narrow band width of 30 nm. Samples of protein concentrations between 1 and 10 mg/ml can be used and no purification or concentration steps after labelling are necessary. Sufficient for 500 lanes or 1250 lanes no matter what size of gel - mini, wide or large format - you use Lager