LB Medium (Lennox), Powder 20 g for 1 liter medum

For cultivation of E. coli in molecular biology.10 g/l Tryptone5 g/l Yeast extract5 g/l NaClFor making 1 L liquid medium suspend 20 g in 900 ml distilled water, adjust the pH to 7.0 with approximately 0.2 ml of 5N NaOH, fill up to a final volume of 1 L with deionized water and sterilize by autoclaving. Lager 
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