Suited for preparation of continuous and discontinuous density gradients. For stabilization of membrane-bound particles, isolation of lymphocytes. Dialyzable material (including NaCl) max. 1 %. Better osmotic properties than sucrose, it preserves functional and morphological integrity and does not penetrate biological membranes.FICOLL™ 400 is a neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution. Concentrations up to 50 % (w/v), covering a density range up to 1.2 g/ml, can be obtained without exceeding normal osmolarity.Suitable for producing density gradients for separation by centrifugation or sedimentation at unit gravity (especially valuable for the separation of cells which are sensitive to centrifugation and for separating cells of similar density but different size). It is also used as a hapten carrier, and in electrophoresis, hybridization and cryopreservation.